Enzymatic synthesis using glycoside phosphorylases
نویسندگان
چکیده
منابع مشابه
N-glycan degradation by bacterial glycoside phosphorylases 1 Role of glycoside-phosphorylases in mannose foraging by human gut bacteria*
In order to metabolise both dietary fibre constituent carbohydrates and host glycans lining the intestinal epithelium, gut bacteria produce a wide range of carbohydrate active enzymes, of which glycoside hydrolases are the main components. In this study, we describe the ability of phosphorylases to participate in the breakdown of human Nglycans, from an analysis of the substrate specificity of ...
متن کاملDevelopment and application of a screening assay for glycoside phosphorylases.
Glycoside phosphorylases (GPs) are interesting enzymes for the glycosylation of chemical molecules. They require only a glycosyl phosphate as sugar donor and an acceptor molecule with a free hydroxyl group. Their narrow substrate specificity, however, limits the application of GPs for general glycoside synthesis. Although an enzyme's substrate specificity can be altered and broadened by protein...
متن کاملRole of glycoside phosphorylases in mannose foraging by human gut bacteria.
To metabolize both dietary fiber constituent carbohydrates and host glycans lining the intestinal epithelium, gut bacteria produce a wide range of carbohydrate-active enzymes, of which glycoside hydrolases are the main components. In this study, we describe the ability of phosphorylases to participate in the breakdown of human N-glycans, from an analysis of the substrate specificity of UhgbMP, ...
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Phenylpropanoid glycosides (PPGs) are natural compounds present in several medicinal plants that have high antioxidant power and diverse biological activities. Because of their low content in plants (less than 5% w/w), several chemical synthetic routes to produce PPGs have been developed, but their synthesis is a time consuming process and the achieved yields are often low. In this study, an al...
متن کاملDisaccharide Synthesis by Enzymatic Condensation of Glucose: Glycoside Linkage Patterns for Different Fungal Species
Four enzyme preparations produced by fungal species belonging to different genera (Aspergillus niger, Corynascus sp., Penicillium verruculosum, Trichoderma reesei) were used for synthesis of disaccharides by D-glucose (60% w/v) condensation catalyzed by -glucosidase. Effects of pH and temperature on the disaccharide synthesis were studied, and glycoside linkage patterns for enzymes from differe...
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ژورنال
عنوان ژورنال: Carbohydrate Research
سال: 2015
ISSN: 0008-6215
DOI: 10.1016/j.carres.2014.06.010